The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
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How to use the Tm calculator The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The annealing temperature gradient should start with temperature 6-10☌ lower than annealing temperature generated by the calculator and increased up to the extension temperature (two-step PCR). If necessary, use a temperature gradient to further optimize and empirically determine the ideal annealing temperature for each template-primer pair combination. T m values, annealing temperature, and other data are automatically generated. To use the calculator select your DNA polymerase, type in or paste your primer sequences, and provide your final primer concentration. The parameters were adjusted on a set of primers seeking to maximize specificity and retain high yields. The modified Allawi & SantaLucia's thermodynamics method (1) is used for T m and annealing temperature calculation of reactions with Platinum SuperFi, Phusion and Phire DNA Polymerases.
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The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. Based on these results, a simple model is put forward for the prediction of the final state of the stress and distortion in cold-sprayed flat components. It has been found that the heat input and the associated thermal history have a major influence on the final distortion and the residual stress, to an extent that the in-plane stress can in some cases change from compressive to tensile. In addition, the amount of heat input as a key parameter in the build-up of the residual stress is examined.
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The influence of various parameters such as the dimensions and elastic properties of the coating and the substrate on the residual stress are analyzed. In this research, analysis of residual stresses in cold-sprayed deposits is performed by analytical and numerical modeling. In cold-sprayed coatings, in which particles impact the substrate at high velocity in solid state, in-plane residual stresses are usually conceived to be compressive. Abstract Residual stress in coatings has significant effect on their performance.
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Thermo-Calc is a powerful software package used to perform thermodynamic and phase diagram calculations for multi-component systems of practical importance.